《植物生理学报》 2018, 54(6): 1055-1063

银杏MADS-box基因家族的表达及系统发育分析

董金金^1,2, 刘伟², 李萌^1,2, 王俊燚², 何崇单², 蔡雨蒙², 王启业², 王义强^1,2,*

¹中南林业科技大学经济林培育与保护教育部重点实验室, 长沙410004; ²中南林业科技大学林业生物技术湖南省重点实验室, 长沙410004

通信作者：王义强；E-mail: wangyiqiang12@163.com

摘　要：

MADS-box基因是真核生物中一类编码转录调控因子的基因家族, 在植物花器官发育中发挥重要的调控作用。为了探究MADS-box家族基因在银杏花发育中的功能, 本文对银杏花芽分化4个时期的样品进行转录组测序, 筛选其中的MADS-box家族基因, 利用生物信息学方法对筛选到的基因进行表达模式、蛋白结构、细胞定位和系统进化分析。结果显示: 共得到15个银杏MADS-box家族基因。表达分析表明, 目标基因根据表达模式可以分为3种类型。分析目标基因的编码序列显示, 15个银杏MADS-box蛋白的主要构件均为α-螺旋和无规则卷曲; 亚细胞定位预测主要在细胞核内; 所有表达产物均包含MADS结构域。聚类分析显示, 银杏MADSbox基因家族可分为6个亚类。银杏MADS-box基因家族中的newGb2734、Gb38883、Gb28587和Gb33168可能在银杏开花调控中发挥重要作用; Gb16301可能是银杏花器官的发育过程中的关键基因。

关键词：银杏; MADS-box基因家族; 开花调控; 花器官发育

收稿：2017-12-22   修定：2018-05-15

资助：国家自然科学基金(31570682)和国家重点研发计划(2017YFD0600701)。

Gene expression and phylogenetic analysis of MADS-box family genes in Ginkgo biloba

DONG Jin-Jin^1,2, LIU Wei², LI Meng^1,2, WANG Jun-Yi², HE Chong-Dan², CAI Yu-Meng², WANG Qi-Ye², WANG Yi-Qiang^1,2,*

¹Key Lab of Non-wood Forest Nurturing and Protection of National Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China; ²Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha 410004, China

Corresponding author: WANG Yi-Qiang; E-mail: wangyiqiang12@163.com

Abstract:

The MADS-box gene is a family of genes that encode transcriptional regulators in eukaryotes and plays an important regulatory role in floral development. In order to study the function of MADS-box gene in G. biloba, We sequenced the transcriptome of G. biloba, including four stages of flower bud differentiation, and screened the MADS-box family of G. biloba. Expression pattern, sequence signature, subcellular location and phylogenetic analysis were analyzed by the bioinformatics methods. The results showed that, 15 G. biloba MADS-box family genes were obtained. According to the different types of expression patterns of differentially expressed genes, the target gene could be divided into 3 types. Analysis of the coding sequence of the target gene showed that the main components of 15 G. biloba MADS-box proteins were α-helix and nonregular coil. The subcellular localization was mainly in the nucleus. All the expressed products contained MADS domain. The newGb2734, Gb38883, Gb28587 and Gb33168 in the MADS-box gene family of G. biloba might play an important role in the flowering regulation of G. biloba. Gb16301 might be the key gene in the developmental process of G. biloba.

Key words: Ginkgo biloba; MADS-box gene family; expression analysis; phylogenetic analysis